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We effectively determined the distribution coefficients of varied natural compounds regarding to their buildup on the water/bonded layer interface and into the bonded layer from bulk water making use of surface-bubble-modulated fluid chromatography with octadecyl- and phenyl hexyl-bonded silica columns. The water/phenyl hexyl-bonded layer user interface accumulates natural substances never as as compared to water/octadecyl-bonded layer interface due to