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Immobilization of enzyme based on combination of adsorption and cellulose derivative membrane coating was established in this work for the first time. Laccase, a commonly used enzyme in varied fields, was chosen as the model enzyme to demonstrate this method. After investigating operational conditions, the optimal process was obtained as follows diatomite or HPD-417 as the adsorption carrier, 0.5% (w/v) methylcellulose (40,000~50,00 acetone solution as the coating solution, 0.75% (w/v) polyethylene glycol or maltose as the protective
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