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Compared with those of the oocytes in the NC group, the early apoptosis rate significantly decreased (P less then .01), whereas both the high-quality cleavage embryo and blastocyst rates significantly increased (both P less then .05) in the oocytes of the 10-9 M-MC group. Finally, single-cell RNA sequencing and immunofluorescence results revealed that aquaporin (AQP) 1/2/11 gene expression and AQP1 protein expression were upregulated in the MC group. Therefore, these results suggest that MLT can improve the effect of cryopreservation on hu
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