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A moderate two-step pretreatment method was investigated to improve the enzymatic saccharification of bamboo residues. SEM and FTIR were employed to characterize the structure changes. Fed-batch enzymatic saccharification was performed to obtain high concentration of fermentable sugar. Bamboo was impregnated at low severity of conditions (room temperature, 2% H2SO4 or 2% NaOH, 48 h) to initially alter the structure of bamboo, and then further pretreated by steam explosion at 1.0 MPa for 6 min. The highest delignification of 51% and the
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