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In this chapter, we explain a detailed procedure to separate your lives OmpA-like proteins, which might be accustomed additional progress the biological researches of OmpA-like proteins.The objective for this section is provide a detailed purification protocol for the surface-layer (S-layer) glycoproteins associated with periodontal pathogen Tannerella forsythia. The process requires detergent based solubilization of the microbial S-layer followed by cesium chloride gra