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BACKGROUND How to select healthy reference subjects in deriving 99th percentiles for cardiac troponin assays still needs to be clarified. To assist with global implementation of high sensitivity (hs)-cardiac troponin (cTn) I and hs-cTnT assays in clinical practice, we determined overall and sex-specific 99th percentiles in 9 hs-cTnI and 3 hs-cTnT assays using a universal sample bank (US. METHODS The Universal Sample Bank (US comprised healthy subjects, 426 men and 417 women, screened using a health questionnaire. Hemoglobin A1c (>