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HAM includes epithelial cells, fibroblasts and mesenchymal stem cells. Consequently, the effective decellularization of HAM with just minimal adverse effects on its ECM components is very important to avoid graft rejection and shows improved overall performance. To date, a few approaches have already been performed for decellularization of HAM, which is primarily based on enzyme-, detergent- or technical procedures with various ranges of success. Right here, we explain a syste