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Aberration in leptin expression is one of the most frequent features in the onset and progression of obesity, but the underlying mechanisms are still unclear and need to be clarified. This study investigated effects of the absence of gut microbiota on body weight and the expression and promoter methylation of the leptin. Four- to five-week-old male C57 BL/6J germ-free (GF) and conventional (CV) mice were fed either a normal-fat diet (NFD) or a high-fat diet (HFD) for 16 weeks. Six to eight mice from each group, at 15 weeks, were admini
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