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Compared with existing approaches, the proposed method exhibits some merits such as s high throughput, good sensitivity and eco-friendliness. Most of important, the MBA/ITMA for on-site preparation avoids the storage and transportation of large volumes of waters, and guarantees the analytical accuracy of studied AAs.Abnormal DNA glycosylases are concerned with the aging process as well as numerous pathologies in humans. Herein, a sensitive fluorescence method utilizing target-induced ligation-dependent tricyclic cascade amplification rea