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By simply altering the sequences of the target recognition sites in MNAzyme, we applied the assay for two types of nucleic acids (long strand DNA and short strand RNA), malaria DNA and miRNA-10b. With increasing the target concentration, the signal intensity of each assembled particle decreases, but the frequency of assembled particle pulse increases. Both targets could be quantitatively detected from 0.1 to 25 pmol L-1 with high specificity in serum samples. The developed MNAzyme-SP-ICP-MS assay possesses simple operation in a homogeneous