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p value ≤0.25 in the bivariate were fitted to a multivariable regression model with a 95% confidence interval. Results Among the 263 HIV-positive patients, 237 (90.11%) were included in the final analysis. The overall prevalence of anemia was 26.2%. Factors that were significantly associated with anemia were past opportunistic infections, patients being in WHO clinical stage III and IV, and a BMI less then 18.5. Conversely, those patients who took anti-TB medication were less likely to have anemia. Conclusion Our study shows that the se