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TECHNIQUES Here, we sought to handle this need by developing a model to analyze metastatic breast cancer cell-microglial interactions making use of intravital imaging coupled with ex vivo electrophysiology. We implanted an optical window regarding the parietal bone tissue to facilitate observance of cellular behavior in situ within the outer cortex of heterozygous Cx3cr1GFP/+ mice. RESULTS We detected GFP-expressing microglia in Cx3cr1GFP/+ mice up to 350 μm below the window with