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The results are useful for the development of algae biotechnology and the possibility of using EMI as a factor which regulates the production of biomass and biohydrogen by green microalgae.A rapid, highly sensitive and simple high-performance liquid chromatographic-tandem mass spectrometric (LC-MS) assay is developed and validated for the quantification of leuprolide a Gonadotropin Releasing Hormone analog (GnRH) in human plasma. Moreover, various parameters of the method stability are determined. After the addition of stable isotope (i