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The addition of CHX or povidone-iodine did not affect the alkalinity of calcium hydroxide. This study concludes that Ca(OH) + 2% CHX are effective against . Combinations of calcium hydroxide and 5% povidone-iodine showed better antibacterial effect than calcium hydroxide and saline. Ca(OH) + saline was ineffective against . This study concludes that Ca(OH)2 + 2% CHX are effective against E. faecalis. Combinations of calcium hydroxide and 5% povidone-iodine showed better antibacterial effect than calcium hydroxide and saline. Ca(OH)2 +