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Lastly, we connect the inferred patterns of recombination with the frequencies of major inversions segregating in natural Drosophila populations. © The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution.INTRODUCTION Recent malicious use of chemical warfare agents (CWAs) is a reminder of their severity and ongoing threat. One of the main categories of CWAs is the organophosphate (OP) nerve agents. Presently, there is an urgent need to identify and evaluate OP nerve agent