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This study aimed to look at the Mn and Cd focus from various samples. Sampling ended up being done twice following the period of 6 months during 2018; five various websites from Chakwal (Pidh, Tobar, Ratoccha, Kalar Kahar Road, Choa Saiden Shah and Chakwal path, Choa Saiden Shah) were selected. Thirty samples of earth, forage (Acacia nilotica, Ziziphus nummularia, and Acacia modesta), and blood had been gathered