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Here, the entire chloroplast genome sequence of M. ruthenica had been reported. The chloroplast genome is 126,939 bp in total. This chloroplast genome does not have any inverted perform (IR) regions, which is common within the household Fabaceae. The M. ruthenica chloroplast genome encodes 107 genetics, including 73 protein-coding genes, 30 tRNA genes, and 4 rRNA genes. Phylogenetic evaluation outcome strongly suggested that M. ruthenica is a distinct lineage in Medicago
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