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In tumors, PD-L1 appearance in cancer cells had been notably greater in HER2-positive samples than in TN normal-like tumors (p = 0.01, while the portion of PD-L1-positive TILs (p = 0.037). PD-L1 gene sequencing identified two heterozygous mutations in exon 4 (A245T; V252M) and another in exon 5 (T267S). To sum up, results support the use of spontaneous feline mammary carcinoma as a model for individual breast cancer tumors and declare tha