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To assess the seroprevalence of anti-SARS-CoV-2 IgG among health careworkers (HCWs) in our university hospital and verify the risk of acquiring the infection according to work area. Cross-sectional study. Monocentric, Italian, third-level university hospital. All the employees of the hospital on a voluntary base, for a total of 4055 participants among 4572 HCWs (88.7%). Number of anti-SARS-CoV-2 positive serology according to working area. Association of anti-SARS-CoV-2 positive serology to selected variables (age, gender, country of o