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S.), more often at the proximal stomach (p  less then  0.05). In human ex vivo model, traditional surgical suture (i.e. running hand-sewn) produced an effective temporary closure, with superior resistance to increasing volume and pressure. How this may impact on clinical LSG outcomes needs further evaluations and was not the object of this study.The development of an effective approach to improve low-temperature nitrification of domestic wastewater remains an important issue that needs to be urgently addressed. This study was intended to verify th