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They donate to invertebrate deterrence. A broad diversity of ecdysteroids takes place in phytoecdysteroid-containing plant species, often in large quantities. Ecdysteroids display possibly useful pharmaceutical activities in mammals. OBJECTIVES Establish reversed-phase high-performance liquid chromatography with combination mass spectrometry (RP-HPLC-MS/MS) and RP-HPLC-DAD-MS (diode range detector size spectrometry) options for the separation, recognition and quantificati