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In this context, we designed a computer device that may rotate instantly in the confines of a specific dark field, and by using this, we could record [Ca2+ ]cyt dynamics right after flowers have been turned to cause mechanical stimulation. This tool can facilitate the study of perception and early sign transduction in response to mechanical stimulation on a large scale centered on [Ca2+ ]cyt responses. © 2020 Wiley Periodicals LLC. Fundamental Protocol 1 Detection of background luminance sign
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