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The evolved SPE-HPLC-MS/MS treatment (within the sewage-based epidemiology strategy) permitted when it comes to multiple, selective, very painful and sensitive, precise (recoveries ≥ 80.1%) and accurate (CV ≤ 8.1%) determination of new and classic psychoactive substances in wastewater samples. This study is characterized by brand new medical elements, especially in regards to the freeze-thaw and post-preparative stability of this selected psychoactive substances. This is the f
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