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Recent functional studies have reported that amygdala and anterior cingulate cortex (ACC) dysfunction is a reproducible and good biomarker of major depressive disorder. When we use the activation of these regions as biomarkers of major depressive disorder, a short and simple stimulation paradigm could be preferable to reduce the burden on patients. However, negativity bias, which is the phenomenon by which negative stimuli are processed noticeably faster than positive stimuli, might affect the activation of these regions in the short an