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Nonetheless, the foundation of pyrK continues to be unknown. In this research, based on our observance in vitro that pyrK occurs in oxidized low-density lipoprotein and in modified proteins with oxidized polyunsaturated essential fatty acids, we used LC-electrospray ionization (ESI)-MS/MS along with a well balanced isotope dilution approach to do activity-guided separation of energetic particles in oxidized lipids and identified glycolaldehyde (GA) as a pyrK origin. Outcomes from m
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