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The current research defines a unique duplex PCR protocol, that is a marked improvement from the traditional PCR methodology, improved by presenting the actin gene as an endogenous control gene. After adjusting the mosquito share dimensions , DNA extraction, and WbCx PCR duplex design, we achieved a dependable and painful and sensitive molecular xenomonitoring protocol. This assay surely could eradicate 5% of false unfavorable examples and detected significantly less than o