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1(3) cm-1. Ab initio computational studies reveal a different type of magnetic anisotropy to be present in the two crystallographically - independent Co centers in 5-II.Phen-DC3 is among the most commonly used G-quadruplex (G4)-stabilizers in vitro and in cells. Here, we show that the G4-interactive binding interactions enable one to tune the optical properties of Phen-DC3 allowing the detection of G4 structures in cancer cells. This work opens up new directions for the use of Phen-DC3 as a selective G4 fluorescent reporter.The detection