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gloeosporioides species complex isolated from avocado in 65%, whereas Bacillus tequilensis A3 supernatant did so in 25% after 3 days post inoculation. B. tequilensis mycoides A1was a producer of proteases, indolacetic acid and siderophores. Preventive treatment using cell-free supernatant of B. mycoides A1, diminished the severity of anthracnose disease (41.9%) on avocado fruit. CONCLUSION These results reveal the possibility of using Bacillus mycoides A1 as a potential biological control agent. This article is protected by copyright.