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After longtime salivary incubation of 12 d, the bactericidal effect started to weaken, but the anti-adhesion and inhibition effect to biofilm development still exist after 24 d of salivary incubation. The application of a totarol coating on implant or abutment surfaces is a promising potential prophylactic approach against peri-implantitis. In this work, tantalum thin films were prepared on titanium substrates by an ion beam sputtering method. Tantalum thin films were irradiated by gamma-ray with different total dose levels. The effect o