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Tunable morphologies and synergistic optical characteristics of plasmonic semiconductor heterostructures inspire many more applications through the edge and planar defects intrinsic in layered MoS2.An impedimetric aptasensor for the detection of endotoxin in a microfluidic chip was proposed, in which the Apt/AuNPs/SPCE sensing surface was fabricated in a screen-printed electrode with good biological activity and stability. The quantitative detection of endotoxin was accomplished by electrochemical impedance spectroscopy (EIS) measurement b