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Two lab-scale MBBRs had been run simultaneously for 116 times under numerous hydraulic retention times (HRTs). The reactors R1 and R2 were full of K5 synthetic carriers and SB, respectively. Through the outcomes, at an optimum HRT of 6 h, ammonia elimination effectiveness and nitrification price were 86.67 ± 2.4% and 1.43 mg/L.h for the R1 and, 91.65 ± 1.3% and 1.52 mg/L.h for the R2, correspondingly. The microbial community analysis showed that the prevalent genera within the nitri