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Alhagi Gagnebin (Fabaceae Hedysareae) is a small genus of shrubs or subshrubs distributed in temperate and tropical regions of Asia, Europe, and Africa. The exact number of Alhagi species in Iran has been disputable. Studies showed that morphology, seed protein, and chromosome characters could not clearly delimitate the species boundaries of the genus. In order to determine species boundaries and the population structure of Alhagi species in Iran, eight labeled inter simple sequence repeat (ISSR) primers were used to screen 22 populatio