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Therefore, this questionnaire may enable researchers to improve this emerging field of research significantly.The accurate classification of microbes is critical in today's context for monitoring the ecological balance of a habitat. Hence, in this research work, a novel method to automate the process of identifying microorganisms has been implemented. To extract the bodies of microorganisms accurately, a generalized segmentation mechanism which consists of a combination of convolution filter (Kirsch) and a variance-based pixel clusterin
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