https://www.selleckchem.com/pr....oducts/Nicotinamide(
The YeaZ protein of V. harveyi was expressed in Escherichia coli and purified. The purified recombinant protein YeaZ exhibited the protease activity. The proteolytic activities with azocasein as substrate were 39130 U mg-1 . The mutation of the amino acid in active sites such as Asp88 , Ser185 and Trp169 were performed. The enzyme activities of the purified mutant proteins with Asp88 -Ala, Ser185 -Leu and Trp169 -Glu were decreased to 24.28%, 35.27% and 41.66%, respectively. The mutant protein with two amino acid resid
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