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del. As such, the results provide new insights for future work on the utilization of 22-HTG in malignant melanoma treatment. Our data indicate that 22-HTG has anti-tumorigenic properties against melanoma cells through the induction of cell cycle arrest, apoptosis and inhibition of invasiveness potential, as observed in the 3D model. As such, the results provide new insights for future work on the utilization of 22-HTG in malignant melanoma treatment. Optimal experimental design theory proposes choosing specific settings in experimental