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However, the developed optosensor exhibited better sensitivity, was faster and easier to operate and can be modified for the enrichment and detection of other target analytes in various sample matrices.Interferon gamma (IFNγ) is a cytokine and an immunochemical marker that can be used for revealing of infectious diseases and especially for distinguishing of viral and some types of bacterial infections. Blood tests for IFNγ are typically based on immunoassays like Enzyme-Linked Immunosorbent Assay (ELISA). In this paper, a biosensor work