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ATP-binding cassette (ABC) transporters play a crucial role in the development of multidrug resistance in diverse cancer entities. Our study was designed to comprehensively analyze the ABC subfamily B (ABC in renal cell carcinoma (RCC) using The Cancer Genome Atlas (TCGA) datasets. We performed systematic survival analyses of ABCB1-10 using the TCGA datasets for clear cell, papillary, and chromophobe RCC. Results were validated via quantitative polymerase chain reaction in a clear cell RCC (ccRCC) cohort containing 152 samples. Afterw