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To simulate hypoxic problems in vitro, melanoma cells A2058, A375 and MeWo were cultured in a chamber with a gas combination of 5% CO2, 94% N2 and 1% O2. One of the cellular lines tested, MeWo cells presented greater survival rates when comparing to A2058 and A375 cells. Co-cultures of L. lactis articulating GFP or mCherry and MeWo cells disclosed that L. lactis effortlessly express the transgenes under hypoxic problems. Moreover, multispectral optoacoustic tomography (MSOT), and near in
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