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Three-dimensional (3D) cell cultures within gels are used to examine physiological reactions between cells, including bacteria and macromolecules such as enzymes. Using non-denaturing electrophoresis, an anionic Coomassie Brilliant Blue (CB dye successfully bound to enzymes such as trypsin and lysozyme, and reacted with a protein and a bacterium within a gel. Both CBB-bound trypsin and lysozyme retained their enzymatic activities and migrated toward the anode in non-denaturing electrophoresis. CBB-bound trypsin successfully digested