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Copyright © 2020 Hang Shi et al.This study is designed to evaluate the probiotic properties of Lactobacillus brevis (L. brevis) KU200019 and the synergistic activity with prebiotics on antimicrobial activity, together with possible application as an adjunct tradition in fermented milk products. The commercial strain, L. brevis ATCC 14869 was used as guide stress. L. brevis KU200019 was showed higher viability in simulated gastric (99.38±0.21%) and bile (115.10±0.13%) prob