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In this work, the general ion mobility of a few natural substances and their 13C-labeled isotopologues is examined in a higher Kinetic Energy Ion Mobility Spectrometer (HiKE-IMS) at large decreased electric areas as much as 120 Td, that allows the investigation of nonclustered ion types and therefore enables an audio investigation regarding the mobility shift of isotopologues. The results reveal that the measured general ion mobilities of isotopologues having the same e