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2%. The best cutoff value was at 16.6% in our population. The performance of the ADNEX model in differentiating benign and malignant tumors was excellent. The performance of the ADNEX model in differentiating benign and malignant tumors was excellent.Melanoma is considered to be one of the most aggressive human tumors. Thus, early molecular diagnosis with risk factor stratification could be an efficacious strategy to increase the survival rates in affected patients. Murine cell lines B16-F1, B16-4A5, and S91 clone M3 are the ones most