https://www.selleckchem.com/
INTRODUCTION The first lineage separation in mammalian development occurs when totipotent cells of the zygote give rise to the inner cell mass and the trophectoderm. The lineages are strictly separated by an epigenetic barrier. In vitro derivatives of these lineages embryonic stem cells (ESC) and trophoblast stem cells (TSC) are used to study the requirements needed to overcome the barrier in ESC to TSC conversion approaches. METHODS Different combinations of TSC transcription factors were induced in ESC for three days. Cells were kept in TS medium with fet
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