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The characteristics of these methods are described in relation to their application in Raman imaging of the cell. Furthermore, we summarize the software that can facilitate the implementation of these methods. Through its careful selection and application, data mining can act as an essential tool in the exploration of information-rich Raman spectral data.Production-scale fermentation processes in industrial biotechnology experience gradients in process variables, such as dissolved gases, pH and substrate concentrations, which can potent