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The performance was confronted with that of a traditional expression cassette harboring the same promoter and gene of interest but lacking the genetic parts for increased expression efficiency. The optimized expression cassette granted higher protein abundance independently of the expression basis or promoter used proving its value for applications requiring high protein abundance. Copyright © 2020 Neves, Vos, Blank and Ebert.Objective The evaluation of the disease severity in hip osteoarthritis (OA) patients being currently based on s