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WBCs on Day 21 of hypothermic storage, then stored and prepared on Days 7, 14, and 21 to acquire residual WBCs to research the impact of hypothermic storage to their viability and proliferative capability. Viability of residual WBCs was evaluated by staining with annexin V and an antibody cocktail for flow cytometry analysis. Proliferative capability ended up being considered by putting carboxyfluorescein diacetate succinimidyl e
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