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We found MRgFUS acutely enhanced plasma cfDNA (2.6±1.2 fold, p0.01, Wilcoxon signed-rank test), neuron-derived extracellular vesicles (3.2±1.9 fold, p0.01), and brain specific protein S100b (1.4±0.2 fold, p0.01). Further comparison of the cfDNA methylation profiles suggests a signature that is disease and post-BBB opening specific, in keeping with our hypothesis. We also found cfDNA mutant copies of isocitrate dehydrogenase 1 (IDH1) increased, although this was in only one patient known to harbour the tumor mutation. This fir
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