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A very nucleobase-discriminating metalated nucleoside analogue, 3-fluoro-2-mercuri-6-methylaniline, was integrated into an oligonucleotide molecular beacon. Fluorescence emission spectra were calculated following the addition of four different complementary strands, where the nucleobase opposite the metalated analogue differs. The fluorescence results revealed a definite binding selectivity at room-temperature, within the purchase GTCA. The selectivity is founded on different affinities