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Make an effort to investigate the proteomic and metabolomic profiles of human aortic endothelial cells (HAECs) subjected to insulin, normal glucose (NG), high glucose (HG) or its hyperosmolar control high mannitol (HM), and to analyze whether and exactly how HG or HM may market insulin opposition. METHODS AND RESULTS We exposed HAECs to HG and HM in shorter (3 h) and longer-term experiments (24 h), followed closely by insulin treatment plan for 45 min. Label-free proteomics and community evaluation showed a downre