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The NC group had significantly lower mean ECV than the PAD group (12.71% vs. 31.92%, respectively, p less then 0.001). In the PAD group, the mean ECV was slightly lower in patients with collateral vessels than in those without (26.58% vs. 34.88%, respectively, p = 0.047). CONCLUSION Evaluation of skeletal fibrosis in PAD using ECV is feasible. ECV can help identify PAD patients with collateral vessel formation and lay the foundation for future research in PAD management. KEY POINTS • Steady-state equilibrium for ECV measurement of the